1. Plant Biotechnology: An Overview; 2. Culture Media and Culturing of Plant Cells ;  3. Tissue Culture, Totipotency,  Organogenesis, Embryogenesis and Wide Hybridization  ;  4. Protoplast Isolation, Culture, Regeneration and Fusion ;  5. Applications of Tissue Culture: I. Clonal Micropropagation;  6. Applications of Tissue Culture: II. Virus Elimination and Production of Disease Free Plants;  7. Applications of Tissue Culture: III. Production of Secondary Metabolites;  8. Applications of Tissue Culture: IV. Production and Uses of Haploids;  9. Applications of Tissue Culture: V. Conservation of Germplasm;  10. Recombinant DNA Technology: I. Restriction Endonucleases, Restriction ;   Mapping and Cloning / Expression Vectors;  11. Recombinant DNA Technology:  II. Chimeric DNA (Gene Constructs),  Molecular Probes and Gene Libraries;  12. PCR and Microarrays ;  13. Methods for Gene Transfer (Transgenic Plants);  14. Transgenic Crops: I. Resistance to Biotic Stresses (Bt-Cotton and Round-Up Ready Soybean);  15. Transgenic Crops: II. Resistance Against Abiotic Stresses;  16. Transgenic Crops: III. Crop Productivity and Nutritional Quality (Including Flaver Savr Tomato and Golden Rice);  17. Transgenic Crops: IV. Improved Horticultural and Ornamental Plants;   (Including Blue Coloured Moondust Carnation and Roses);  18. Transgenic Crops: V. Molecular Farming/Pharming (Including Edible ;   Vaccines, hGH, Humulin and Industrial Enzymes);  19. Transgenic Crops: VI. Biosafety, Field Testing and Commercialization;  20. Bioremediation  (Including Phytoremediation)